Fig. 1: An interaction between RECQ4 and RPA. | Nature Communications

Fig. 1: An interaction between RECQ4 and RPA.

From: Recognition and coacervation of G-quadruplexes by a multifunctional disordered region in RECQ4 helicase

Fig. 1

a Schematic representation of domain organization across the RecQ helicase family. b Immunoprecipitation of EGFP-RECQ4-WT or EGFP from whole cell lysates by GFP-trap beads. Samples were run on SDS-PAGE gel and immunoblotted on nitrocellulose membrane with indicated antibodies. The gel is a representative image of two independent experiments. c The topology of the RPA-RECQ4(Sld2-like) association was determined by NMR to detect physical interactions between segments. RPA modules are annotated as DNA or protein binders. For the RECQ4 fragment, RPA binding was further refined and located on RSM. Source data are provided as a Source Data file.

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