Fig. 3: RSM forms electrostatically driven high-affinity complexes with DNAs. | Nature Communications

Fig. 3: RSM forms electrostatically driven high-affinity complexes with DNAs.

From: Recognition and coacervation of G-quadruplexes by a multifunctional disordered region in RECQ4 helicase

Fig. 3

a 1H-15N HSQC spectra of 50 μM RSM titrated with double-stranded 10mer DNA (ds10). Some chemical shift perturbations (CSP) are indicated with arrows. Inset shows the crosspeaks of tryptophan sidechains. b CSPs of RSM residues induced by 4× molar addition of ds10. c Binding of RSM (dark color, 70 mM ionic strength; light color, 500 mM ionic strength) to ss10, ds10, HT, or CEB1 DNA monitored by FA measurements. n = 3 independent experiments; data are means ± s.d. d Effect of ionic strength on RSM binding to ds49 or CEB1 DNA quantified in EMSA experiments (see Supplementary Fig. 7f). n = 3 independent experiments; data are means ± s.d. Source data are provided as a Source Data file.

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