Fig. 5: RSM-G4 recognition and coacervation. | Nature Communications

Fig. 5: RSM-G4 recognition and coacervation.

From: Recognition and coacervation of G-quadruplexes by a multifunctional disordered region in RECQ4 helicase

Fig. 5

sRSM interaction with (a) parallel and (b) hybrid G4 was analyzed by ITC experiments (100 μM RSM), CD measurements (10 μM G4), or NMR titrations (50 μM G4). Imino chemical shift perturbations were quantified at sRSM:G4 ratio of 0.75:1 and color mapped in the schematic diagrams. G4 sequences are indicated. c RSM (10 μM), parallel T95-2T G4 (10 μM) or their mixture (10 μM each) were analyzed for droplet formation using DIC microscopy. The addition of 500 mM NaCl dissolves the droplets. Images are representative of three independent experiments. d Droplet formation and colocalization of 5 mol % FITC-labeled RSM (10 μM) and 5 mol % Cy3-labeled CEB1 G4 (10 μM). Images are representative of three independent experiments. e Droplet formation (upper panels) and colocalization (lower panels) of 5 mol% FITC-labeled RSM with preformed droplets. Images are representative of two independent experiments. In all images scale bar = 1 μm. Source data are provided as a Source Data file.

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