Fig. 7: Analysis of G4 structure inside the droplets using Thioflavin T probe. | Nature Communications

Fig. 7: Analysis of G4 structure inside the droplets using Thioflavin T probe.

From: Recognition and coacervation of G-quadruplexes by a multifunctional disordered region in RECQ4 helicase

Fig. 7

a Thioflavin T (ThT, 500 nM) was mixed with T95-2T G4 (10 μM) or ssDNA−18mer (10 μM) in the absence or presence of RSM (10 μM) and the bulk fluorescence intensity was quantified. n = 3 independent measurements; data are means ± s.d. b Turbidity measurements of samples used for ThT fluorescence quantification in (a). Turbidity was measured as light absorbance at 600 nm. n = 2 independent measurements. c Phase separation microscopy of RSM (10 μM) mixed with T95-2T G4 or ssDNA−18mer (10 μM). ThT was added to a final concentration of 500 nM and the ThT fluorescence signal in the droplets was analyzed by fluorescent microscopy. In all images scale bar corresponds to 1 μm. d Manual quantification of ThT intensity in individual droplets from images in (c), n = 54 droplets for G4 and n = 68 droplets for ssDNA; ****p < 0.0001 (two-tailed Mann–Whitney test). Source data are provided as a Source Data file.

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