Fig. 8: RSM binding to a tetramolecular G4 hinders G4 processing by helicases. | Nature Communications

Fig. 8: RSM binding to a tetramolecular G4 hinders G4 processing by helicases.

From: Recognition and coacervation of G-quadruplexes by a multifunctional disordered region in RECQ4 helicase

Fig. 8

a Binding of increasing RSM concentrations to 40 nM fluorescently labeled tetramolecular G4 (TetraG4) and ssDNA. Formation of RSM-G4 complex is reversed by addition of SDS/proteinase K. Schematic representation of ssDNA and folded G4 is shown on the left. b Quantification of gel image shown in (a); n = 3 independent experiments; data are means ± s.d. c ScPif1 helicase (3.1 nM) effectively unwinds tetramolecular G4 (40 nM), but its helicase activity is inhibited by pre-incubation of TetraG4 with RSM. d Quantification of gel image shown in (c), n = 4 independent experiments; data are means ± s.d. Source data are provided as a Source Data file.

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