Fig. 4: Validation of major hits in diploid cells using VSVΔG/MARVGP.

Western blot analysis of the levels of VSV-M protein in various clones of A549 KO cells targeting CCZ1 (a), RAB7 (b) and NPC1 (c), 48 h post infection with rVSV-GP-MARV. Western blot showing the KO of CCZ1, NPC1 and Rab7 are shown in Source data linked to this manuscript. d Survival ratio of mutant (unstained) to WT (GFP) A549 after infection with VSVΔG/MARVGP. The bar graph is representative of 3 replicates. The bar graphs show the change in percentage compared to mixed, uninfected population at each time point. The green bars are on a negative y-axis representing a growing decrease in GFP⁺ cells (WT cells) in the mixed, infected population compared to mixed non-infected population. The blue bars on the other hand, is on a positive y-axis showing a growing increase in GFP^- (KO cells) cells in the mixed, infected population compared to mixed non-infected population. Gating strategy is presented in Supplementary Fig. 3. Relative level of infection of CCZ1 KO (e), MON1A KO (f), MON1B KO (g) and respective transcomplemented (Tr) cells to control cells infected with VSVΔG/MARVGP. Error bars represent mean ± SD, n = 3 biologically independent replicates. *P < 0.05, **P < 0.01, ***P < 0.001; two-tailed Student’s t-test. Exact p values and Source data are provided as a Source Data file.