Fig. 8: Effect of the loss of CCZ1 on virus trafficking in the endosomal pathway. | Nature Communications

Fig. 8: Effect of the loss of CCZ1 on virus trafficking in the endosomal pathway.

From: Identification of CCZ1 as an essential lysosomal trafficking regulator in Marburg and Ebola virus infections

Fig. 8

a Microscopy of CCZ1 of DQ-Red BSA treated cells. In CCZ1 KO cells, DQ-Red BSA doesn’t reach lysosomes (Scale 10 µm). Pictures are representative of 3 independent experiments. b CCZ1 KO leads to a blocking of VSVΔG/MARVGP in vesicles (Green: VSV-M protein, Blue: DAPI). Pictures are representative of 3 independent experiments. c Colocalization of VSV-M protein with the early endosome marker Rab5 in VSVΔG/MARVGP infected wild-type and CCZ1 KO A549. Green: VSV-M protein, Red: Rab5, Blue: DAPI. (Left: level of colocalization; Right: confocal microscopy images). All single channels are presented in supplementary Fig. 5. Error bar represent mean ± SD, n = 6 cells. ****P < 0.001; two-tailed Student’s t-test. Exact p values and Source data are provided as a Source Data file.

Back to article page