Fig. 5: OPA1 KO alters the levels of respiratory complex subunits and formation of supercomplexes. | Nature Communications

Fig. 5: OPA1 KO alters the levels of respiratory complex subunits and formation of supercomplexes.

From: The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury

Fig. 5

a The heatmap of respiratory complex subunits. Uniprot IDs are shown. b Volcano plot of respiratory complex subunits. Proteins with a statistically significant change are in the unshaded area. Q59J78, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2; Q921H9, cytochrome c oxidase assembly factor 7. c–f Immunoblots of the BNGEs for respiratory complexes. OPA1 KO decreases complex I (c) and complex IV (f), and the complex I-containing supercomplexes [I + III2] and [I + III2 + IV] (c, d, and f). Increased complex II levels in OPA1 KO (e). The numbers in the parentheses are the molecular weights in kDa. Each lane represents mitochondrial extract from different animals. g Quantification of the total levels of complexes I–IV. n = 4 (flox) and 5 (KO) animals per group. Data are presented as mean values +/- SD. Unpaired t test. ****p < 0.0001; ***p = 0.0003; **p = 0.0022. Source data are provided as a Source Data file.

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