Fig. 6: OPA1 KO impairs complex V assembly. | Nature Communications

Fig. 6: OPA1 KO impairs complex V assembly.

From: The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury

Fig. 6

a OPA1 KO significantly impairs the assembly of complex V. Sub-complexes smaller than complex V monomer (V1) are accumulated in OPA1-KO mitochondria. b Quantification of (a). The increases in total complex V (p = 0.0004) and unassembled (p < 0.0001). The deceases in complex V monomer (p < 0.0001), dimer (p = 0.0002), and oligomer (p = 0.0304). n = 4 animals per group. Data are presented as mean values +/- SD. Unpaired t test. c A decrease of mtDNA in OPA1-KO liver. n = 5 animals per group. Data are presented as mean values +/- SD. Unpaired t test. **p = 0.0017. d Decreased mtDNA in OPA1-KO or Mfn-DKO MEFs. n = 5 independent measures per group. Data are presented as mean values +/- SD. Unpaired t test. ****p < 0.0001. e Complex V assembly states in WT, OPA1-KO, and Mfn-DKO MEFs by BNGE immunoblot (anti-subunit c antibodies). f, g Quantification of (e). n = 4 independent experiments per group. Data are presented as mean values +/- SD. One-way ANOVA with Turkey’s multiple comparisons. ***p = 0.0001; **p = 0.0036; *p = 0.0401. Source data are provided as a Source Data file.

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