Fig. 5: EndoS is not catalytically active in the murine model of intraperitoneal (IP) GAS infection.
From: Pathogen-driven degradation of endogenous and therapeutic antibodies during streptococcal infections
Animals were infected by GAS strain AP1 with or without IVIG treatment and blood plasma was harvested after 24 h p.i. Glycopeptide analysis of murine plasma glycoforms for a, IgG1. b IgG2c and (c) IgG3 before and after 24 h IP-infection. d Mass spectrometry-based glycopeptide quantification of human IVIG circulating in mouse plasma at 24 h p.i. (n = 5 mice/condition). Upper whisker extends from the hinge to the largest value no further than 1.5 * IQR from the hinge (where IQR is the inter-quartile range). The lower whisker extends from the hinge to the smallest value, at most 1.5 * IQR of the hinge. e qPCR analysis of the expression of EndoS and IdeS in microbial mRNA isolated from spleens of intraperitoneally or subcutaneously infected mice at 24 h postinfection (n = 5/condition). Statistical significance was assessed by two-tailed Mann-Whitney test, *p < 0.05.
