Fig. 4: Increased growth of JFB organoids infected with SARS-CoV-2.

Dissociated JFB distal intestinal organoids or Vero E6 cells were mock-inoculated or were infected with SARS-CoV-2 at an MOI of 1 or 10, as described above, with NucView® 488, a cell membrane-permeable fluorogenic caspase-3 reporter, added to the medium. a At 48 h post infection, the cells were imaged using fluorescence and phase contrast (brightfield) microscopy. Bat005, p7, scale bars = 50 μm, representative of four experiments. b ImageJ was used to quantitate NucView® fluorescence based on pixel counts in thresholded digital images of manually selected organoids. Individual data points, mean ± SD of one representative (bat002, p7) of four independent experiments with three technical replicates is shown, data were analyzed by ANOVA with Tukey’s multiple comparison test. c Organoid size in SARS-CoV-2-infected organoid cultures after 48 h was determined on brightfield images using ImageJ. Individual data points, mean ± SD of one representative experiment (bat001, p6) of five independent experiments with three technical replicates is shown, data were analyzed by ANOVA with Tukey’s multiple comparisons test. d Number of detected organoids in random brightfield images from mock-infected and SARS-CoV-2 infected JFB organoid cultures (MOI 10, 48 h). Pooled data from four independent experiments (bat001, p6; bat002, p7; bat003, p13; bat005, p5); analyzed using a paired 2-sided Student’s t test. Source data are provided as a Source Data file.