Fig. 1: Aversive air puff (AP)-triggered responses of dorsal CA1 pyramidal cells.
From: Aversive stimulus-tuned responses in the CA1 of the dorsal hippocampus
a Schematics of the recording configuration. Linearized belt indicates the different AP locations during AP epochs. Fluorescent micrograph of a sagittal section showing the tracks of the silicon probe. Mol, molecular layer of the dentate gyrus; Or, stratum oriens; Rad, stratum radiatum; S, subiculum. Scidraw. (2020). mouse running. Zenodo. https://doi.org/10.5281/zenodo.3925913. b Representative location-lap number raster (upper plot) and mean tuning curves in no stimulation and AP epochs (lower plots) of an AP-activated pyramidal cell. White arrowheads and violet bars mark AP locations. Light grey dashed lines separate consecutive no stimulation and AP (4) epochs. c Peri AP firing histograms of putative pyramidal cells from all session (n = 851 units, 18 sessions). d Normalized spike number around the AP stimulation calculated in the 1st, 2nd and 3rd AP epochs. Light (pre) and dark (post) grey lines indicate the control epochs, violet line represents the AP epochs, shaded area corresponds to s.e.m. Red line indicates significant differences in spike count between control and AP epochs (n = 54, 12 sessions, Wilcoxon signed rank test, p < 0.05). e Percentages of AP responsive putative pyramidal cells. aAP, air puff activated; nAP, non-AP responsive; iAP, decreased activity upon air puff stimulation; PC, place cell; nPC, non-place cell. f Two representative examples demonstrating the onset of air puff responses (bin size 20 ms). Inset, bin size 5 ms. Note the remarkably rapid response onset of the second aAP-Pyr cell. g Distribution of the duration (left panel) and latency (right panel) of AP-evoked activation. Latency was quantified as time from air puff TTL onset to the 1st spike in the 1st significant bin at logarithmic timescale. h Firing rate during quiet wakefulness (Q) and movement periods (M). Circles represent individual neurons. 2-way repeated measures ANOVA: Factor A (quiet-mov) F(1779) = 75.94, p < 10−17, Factor B (cell groups) F(3779) = 28.65, p = 1.4*10−17, interactions F(3779) = 13.69, p = 1.03*10−8. Post hoc Tukey test: all cell groups are significantly different from each other except of the aAP-nPC vs nAP-nPC. Box and whiskers correspond to median, quartile and 10-90% range. Note, that we broke the y axis for better visualization. Source data are provided as a Source Data file.
