Fig. 2: Interneuron response to aversive air puff (AP) stimuli.
From: Aversive stimulus-tuned responses in the CA1 of the dorsal hippocampus
a Peri AP firing histograms of putative interneurons from all sessions (n = 216, 18 sessions). b Representative location-lap number raster of interneurons with increasing (upper panel, 2 AP epoch) and decreasing activity (lower panel, 4 AP epoch), in response to AP. Arrowheads mark AP locations. Dashed lines separate consecutive no-stimulation and air puff epochs. c Firing histograms of interneurons, with activation (upper panel) and suppression of activity (lower panel) in response to AP (bin size 20 ms). Inset, bin size 5 ms. d Distribution of the duration of AP-evoked activation. e Distribution of the latency of air puff-evoked activation quantified as time from air puff TTL onset to the 1st spike in the 1st significant bin at logarithmic timescale. f Percentage of putative interneurons with different response types. aAP-IN, increasing activity to air puff (n = 55); iAP-IN, decreasing activity to air puff (n = 68); aiAP-IN, biphasic response to air puff (n = 15); nAP-IN – no response upon air puff stimulation (n = 78). g Theta phase preference of air puff responsive putative interneurons. aAP-IN, increasing activity to air puff (1.3°; 0.2); iAP-IN, decreasing activity to air puff (10.4°; 0.3); aiAP-IN, biphasic response to air puff (356.7°; 0.1); nAP-IN – no response upon air puff stimulation (2.4°; 0.4). (mean angle, circular variance). h Proportion of air puff responsive and non-responsive interneurons in theta peak and theta trough phase groups of interneurons. i Representative example of air puff-activated pyramidal cells converging on an air puff-activated putative interneuron. On each subpanel, peri-air puff firing histogram of the respective neuron is presented. j Summary data about the number of postsynaptic interneurons targeted by air puff-activated PC cells (aAP-PC, n = 87), air puff-activated non-place cells (aAP-nPC, n = 48), non-air puff responsive place cells (nAP- PC, n = 364), and nonair puff responsive, non-PC (nAP-nPC, n = 339). Kruskal-Wallis test H(3) = 124.53, p < 0.05, Dunn-Holland-Wolfe post hoc test: aAP-PC vs aAP-nPC p < 0.05, aAP-PC vs nAP-PC p < 0.05, aAP-PC vs nAP-nPC p < 0.05, aAP-nPC vs nAP-PC p > 0.05, aAP-nPC vs nAP-nPC p < 0.05, nAP-PC vs nAP-nPC p < 0.05. Box and whiskers correspond to median, quartile and 10–90% range. Source data are provided as a Source Data file.
