Fig. 3: Amount of spatial information differentially modulated the effect of aversive stimulation on pyramidal cells versus interneurons.
From: Aversive stimulus-tuned responses in the CA1 of the dorsal hippocampus
a Peri AP firing histograms of putative pyramidal cells (n = 361 from 5 sessions) on predisc sphere (pre-Sph), disc and postdisc sphere (post-Sph). Average peri-AP firing histograms of significantly activated and suppressed pyramidal cells are shown below the histograms, shaded areas correspond to s.e.m. b Proportion of AP-activated pyramidal cells (upper panel, 5 session, mean ± SD) on pre-sphere, disc and postsphere. Pre-sphere: 0.31 ± 0.086, disc: 0.43 ± 0.10, postsphere: 0.23 ± 0.06; one-way-ANOVA F(2,12) = 6.6, *p = 0.012, Tukey’s post hoc test: disc vs. pre-sphere: p = 0,11, disc vs. postsphere: p = 0.009. Suppressed pyramidal cells on pre-sphere: 0.007 ± 0.01, disc: 0.12 ± 0.07, postsphere: 0.010.016; one-way-ANOVA F(2,12) = 13.3, p = 0.0009, Tukey’s post hoc: disc vs presphere: **p = 0.002, disc vs post-sphere: **p = 0.002. Lower panel: Cumulative distribution of pyramidal cell response magnitudes on pre-sphere (black line), disc (violet) and postsphere (grey). Inset highlights the increased probability of AP-evoked suppression in pyramidal cells’ activity on the disc. Kolmogorov-Smirnov test pre-Sph vs disc, **p = 0.003; disc vs post-Sph **p = 6*10−6 c Distribution of the duration of AP-evoked activation of pyramidal cells on pre-sphere (black, upper panel), disc (violet, middle panel) and postsphere (grey, lower panel). For visualization purposes time axis was truncated at 2 s also at (f) panel. d Peri AP firing histograms of putative interneurons (n = 88 from 5 sessions) on presphere (left), disc and post-sphere (right). Average peri AP firing histograms of significantly increased and suppressed interneurons are shown below the histograms, shaded areas correspond to s.e.m. e Proportion of air puff-activated interneurons (upper panel, 5 session, mean ± SD) on presphere: 0.50 ± 0.12, disc: 0.35 ± 0.15 and postsphere: 0.38 ± 0.10; one-way-ANOVA F(2,12) = 2.2, p = 0.15. Suppressed interneurons on presphere: 0.10 ± 0.11, disc: 0.36 ± 0.17, postsphere: 0.09 ± 0.10; one-way-ANOVA F(2,12) = 6.8, p = 0.01, Tukey’s post hoc: disc vs pre-sphere: *p = 0.021, disc vs postsphere: p = 0.018. Lower panel: Cumulative distribution of air puff-responsive putative interneurons’ response magnitude on presphere (black line), disc (green) and postsphere (grey). Kolmogorov-Smirnov test pre-Sph vs disc, **p = 0.0006; disc vs. post-Sph ** p = 0.0007 (f) Distribution of air puff-response durations of interneurons on presphere (black, upper panel), disc (green) and post-sphere (grey, lower panel). Source data of panel b and e are provided as a Source Data file.
