Fig. 6: Aversive air puffs suppress place cell spiking within collocating place fields and augments out of field activity while triggers remapping.

a Peri air puff z-scored firing histograms of putative pyramidal cells if air puff was given inside place field (Infield, left panel, n = 133) or outside place field (Outfield, right panel, n = 133). The color bar between the firing histograms indicates the identity of the corresponding cell: red, aAP, air puff-activated place cell; green, nAP-PC, air puff-non-responsive place cell; blue, iAP, air puff-inhibited place cell. Below the PSTH plots are the averages of the corresponding matrices, shaded error bars correspond to s.e.m. b Z-scored outfield air puff responses as a function of infield air puff responses of putative pyramidal cells (upper panel). Circles represent putative pyramidal cells. Red line marks z-score value of 2 (2 SD). c Summarized data showing z-scored infield and outfield air puff responses (infield: 0.11 ± 1.63, outfield: 0.28 ± 1.1 Wilcoxon signed rank test **p = 0.0005, n = 133 units; middle, box and whiskers correspond to median, quartile and 10-90% range). d Place field distances from the location of air puff stimulus before the stimulus (Pre) versus after the stimulus (Post). Place fields along the diagonal correspond to stable place fields, off diagonal circles are shifted place fields (remapped). Circles along the x axis are the vanishing and along the y axis are the emerging place fields. Air puff responses at different locations are pooled together. Grey histograms show the distribution of emerging, vanishing fields, violet histograms correspond to stabile and remapping place fields. e Correlations of place maps (n = 364 nAP-PC, 3 AP locations) calculated between first and second halves of pre stimulation (Pre), air puff stimulation (AP) and post stimulation epochs (Post). Kruskal-Wallis test H(5) = 126.6, p < 0.005. Dunn-Holland-Wolfe: there are significant differences: * AP-AP vs Pre-Pre; Post-Post and Pre-Post vs Pre-Pre or Post-Post. Box and whiskers correspond to median, quartile and 10-90% range. Source data of panel b-e are provided as a Source Data file.