Fig. 8: Reactivation of air puff selective assemblies.
From: Aversive stimulus-tuned responses in the CA1 of the dorsal hippocampus
a Occurrence of a representative air puff assembly identified by using both AP and non-AP epochs (violet, upper panel) during the session and similar air puff assembly identified by opting out air puff epochs (grey, lower panel) from the same session. Violet rhombi indicate air puff stimulation. For visualization purposes, assembly activation during air puff epochs was removed from the lower panel. b Assembly activation strength before air puff stimulus (Pre) between air puff stimuli (3 air puff stimuli, Inter Event Interval 1 and 2 (IEI)) and after air puff stimuli (Post). Violet circles mark air puff assemblies (n = 6 from 6 sessions) and grey circles correspond to non-air puff assemblies (n = 84 from 6 sessions). (2-way repeated measures ANOVA: Factor A (AP-a – nAP-a), F(1,43) = 0,627, p = 0.433; for Factor B (Pre, IEIs and Post) F(3,129) = 8.679, p = 2.74*10−5; Interaction F(3,129) = 4,784, p = 0.003). There are significant differences between Pre AP-a strength and Post AP-a strength with Tukey post hoc test, *p = 0.021. c Representative air puff assembly reactivation (grey, middle panel) in comparison to the occurrence of ripples (red, upper panel) and speed (orange, lower panel). d Reactivation occurrence of air puff-assemblies (AP-a, n = 7 from 6 sessions) and non-air puff assemblies (n = 84 from 6 sessions) during movement. For testing significance Wilcoxon-Mann-Whitney two-sample rank-sum test was used, p > 0.05. e Ratio of assembly reactivation strength of air puff and non-air puff assemblies during immobility versus movement of air puff and non-air puff assemblies (AP-a, 2.36 ± 1.2, n = 6 from 6 sessions; nAP-a, 1.49 ± 1.26, n = 84 from 6 sessions). For testing significance Wilcoxon-Mann-Whitney two-sample rank-sum test was used, *p = 0.012. f Ripple-triggered average of normalized assembly reactivation strength for both air puff- and non-air puff assemblies, shaded area corresponds to s.e.m. Inset shows the statistically similar normalized reactivation strength of air puff- and non-air puff assemblies in the ±10 ms peri-ripple peak window (AP-a, 0.032 ± 0.025, n = 7 from 6 sessions; nAP-a, 0.030 ± 0.02, n = 84 from 6 sessions Wilcoxon-Mann-Whitney two-sample rank-sum test, p = 0.73). On panels b,d,e and inset of f, box and whiskers correspond to median, quartile and 10-90% range. Source data of panel b,d,e are provided as a Source Data file.
