Fig. 2: PAXT subunits are co-recruited to chromatin.

a Browser shots of RNAPII, ZFC3H1, RBM26, and PAPγ ChIP-seq signal over a representative gene in HeLa cells. A schematic representation of the gene is shown above. b ChIP-seq heatmaps centered on TSSs ± 5 kb and rank-ordered by normalized RNAPII ChIP-seq signal. Normalized ChIP-seq reads of ZFC3H1, RBM26, or PAPγ were plotted respecting the same ranking. Scaled average density profiles of normalized ChIP-seq reads are shown above. c Scatter plots showing the normalized ChIP-seq signal of PAPγ relative to that of ZFC3H1 or RBM26 at the TSS of genes with a high or low occupancy of RNAPII at the TSS, as indicated. d Venn diagram showing the overlap of ChIP-seq peaks of ZFC3H1, RBM26, and PAPγ. P-values were calculated using Fisher’s exact test (^***P < 0.001). e Re-ChIP analysis performed in HeLa cells showing co-localization of ZFC3H1 and PAPγ at the TSS region of the indicated genes. Eluates from an initial ChIP using ZFC3H1, or a control IgG antibody (1st ChIP) were used for PAPγ ChIP or no antibody as a control (re-ChIP). Results of qPCR after 1st and 2nd ChIPs are shown as % of input for 1st ChIP. Data represent mean ± SEM obtained from 3 independent experiments (***P < 0.001, NS indicates not significant, one-sided independent Student’s t-test). Source data are provided as a Source Data file.