Fig. 7: NHP-induced systemic immunity depends on eNAD(P) and its receptor complex.

a NHP-induced systemic immunity in WT and 35 S:CD38 plants. Three lower leaves were infiltrated with 0.25 mM NHP or water (-NHP). Twenty-four hr later, one upper systemic leaf on each plant was inoculated with Psm. Samples were collected at 72 hpi. Bars represent means ± SE (n = 12 independent leaf disks). The NHP-induced systemic immunity was significantly reduced in the 35 S:CD38 transgenic plants (two-way ANOVA with Sidak’s test). The experiment was performed three times with similar results. b NHP-induced systemic expression of PR1, ALD1, and FMO1 in the indicated genotypes. Three lower leaves on each plant were infiltrated with 0.5 mM NHP or water (-NHP). One upper systemic leaf on each plant was collected 24 h later. Bars represent means ± SE (n = 3 independent total RNA samples). Different letters denote significant differences (one-way ANOVA with Tukey’s test; p values are shown in the Source Data file). The experiment was repeated with similar results. c, d NHP-induced systemic immunity in the indicated genotypes. The photo in (d) was taken at 72 hpi. Bars in (c) represent means ± SE (n = 20 independent leaf disks). Different letters denote significant differences (one-way ANOVA with Tukey’s test; p values are shown in the Source Data file). The experiment was performed three times with similar results.