Fig. 1: ZFYVE28 expression was lower in obese-IS subjects but higher in insulin-resistant subjects. | Nature Communications

Fig. 1: ZFYVE28 expression was lower in obese-IS subjects but higher in insulin-resistant subjects.

From: ZFYVE28 mediates insulin resistance by promoting phosphorylated insulin receptor degradation via increasing late endosomes production

Fig. 1

a Test procedure for insulin-sensitive obese (obese-IS) patients, insulin-resistant MetS (IR) patients and normal controls (n = 13 per group). MetS, metabolic syndrome. b, d Volcano plots illustrated the differentially expressed genes in whole blood cells between controls and obese-IS patients (b), as well as controls and MetS patients (d). Compared to controls, ZFYVE28 expression was decreased in obese patients (FC = 0.75, P = 0.011) but increased in insulin-resistant patients (FC = 1.24, P = 0.023). Points corresponding to significantly downregulation and upregulation genes are colored blue and red, respectively. ZFYVE28 is highlighted with a green dot. Fold change is displayed in a log2 scale and P values in −log10 (P value); FC fold change. c, e The expression difference of ZFYVE28 in the remaining samples was further verified by RT-qPCR (87 controls vs. 87 obese-IS patients, 87 controls vs. 87 MetS patients). f–j Gross images (f), body weight (g, h), liver images (i) and HE staining results of liver tissues (j) of mice in the HFD-4w group and HFD-12w group. ND: n = 3; HFD-4w: n = 6; HFD-12w: n = 6 biologically independent mice. Scale bar, 100 μm. k, o Serum insulin levels of mice in the HFD-4w group (k) and HFD-12w group (o). ND: n = 3; HFD-4w: n = 6; HFD-12w: n = 6 biologically independent mice. l, m, p, q GTT and ITT results showed normal glucose tolerance (l) and insulin sensitivity (m) in HFD-4w group mice, as well as impaired glucose tolerance (p) and insulin sensitivity (q) in HFD-12w group mice. ND: n = 3; HFD-4w: n = 6; HFD-12w: n = 6 biologically independent mice. n, r Zfyve28 expression was downregulated in the liver of obese-IS mice in the HFD-4w group (n) but upregulated in the liver of insulin-resistant mice in the HFD-12w group (r). ND: n = 3; HFD-4w: n = 6; HFD-12w: n = 6 biologically independent samples. s–v Western blot assays of Zfyve28 expression in the livers of mice in the HFD-4w group (s) and HFD-12w group (t), and the quantitative analysis results (u, v) are presented. ND: n = 3; HFD-4w: n = 6; HFD-12w: n = 6 biologically independent samples. Data are shown as means ± SD, and P values are determined by unpaired two-tailed Student’s t-test (b–e, g, h, k, n–o, r, u, v) or two-way ANOVA with Fisher’s LSD post hoc multiple comparisons test (l, m, p, q). The exact P values are shown in the figure. Source data are provided as a Source Data file.

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