Fig. 4: Global Zfyve28 knockout improved the insulin sensitivity in mice.

a Construction strategy of Zfyve28 global knockout mice. b, c Zfyve28 was knocked out in KO mice; n = 5 biologically independent samples per group (b). Representative western blot results of three independent parallel experiments are shown (c). d–f Representative western blot results (d) of hepatic proteins in WT and KO mice after insulin injection (2 U/kg body weight) for 30, 60, and 90 min. The quantitative analysis results of three independent parallel experiments (e-f) are shown. g The body weight of KO mice was lower than that of WT mice when fed a HFD; n = 5 biologically independent mice per group. h, i GTT (h) and ITT (i) results of WT mice and KO mice; n = 5 biologically independent mice per group. j, k Global Zfyve28 knockout prevented HFD-induced hepatosteatosis. Representative gross images (j) and HE staining results (k) of livers are presented; n = 5 biologically independent samples per group. Scale bar, 100 μm. l–p Western blot analysis (l) of hepatic protein levels in WT and KO mice. The quantification results (m–p) are shown; n = 3 biologically independent samples per group. Data are shown as means ± SD, and P values are determined by unpaired two-tailed Student’s t-test (b, e–g, m–p) or two-way ANOVA with Fisher’s LSD post hoc multiple comparisons test (h, i). The exact P values are shown in the figure. Source data are provided as a Source Data file.