Fig. 8: Plinabulin treatment impaired insulin sensitivity in mice. | Nature Communications

Fig. 8: Plinabulin treatment impaired insulin sensitivity in mice.

From: ZFYVE28 mediates insulin resistance by promoting phosphorylated insulin receptor degradation via increasing late endosomes production

Fig. 8

a, b HepG2 cells were treated with plinabulin (200 nM) for 24 h and subsequently treated with insulin (1 µM) for different times. Representative western blots of the plasma membrane (pMem), organelle membrane (oMem), cytosol and total INSR (a) and quantitative analysis of three independent parallel experiments (b) are shown. c, f, i Body weights of plinabulin-treated HFD-fed WT (c), KO (f), and LKO (i) mice; n = 5 biologically independent mice per group. d, e Plinabulin treatment impaired insulin sensitivity in HFD-fed WT mice; n = 5 biologically independent mice per group. g, h, j, k Plinabulin treatment markedly impaired insulin sensitivity in HFD-fed KO (g, h) and LKO mice (j, k); n = 5 biologically independent mice per group. Data are shown as means ± SD, and P values are determined by unpaired two-tailed Student’s t-test (b, c, f, i) or two-way ANOVA with Fisher’s LSD post hoc multiple comparisons test (d, e, g, h, j, k). The exact P values are shown in the figure. Source data are provided as a Source Data file.

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