Fig. 3: Response of m⁶A-modified NCOA4 mRNA in PBMCs from non-human primates (NHPs) and human cells exposed to radiation.

a Homology analysis of m⁶A methylated sites and conserved motifs around these sites in Ncoa4/NCOA4 mRNA among different species. Schematic representation of m⁶A sites and correspondingly primers designed to investigate the m⁶A levels of NCOA4 mRNA with exposure to radiation in NHPs (b) and human cells (c). d Validation of primers specificity and measurement of RNA m⁶A levels of NCOA4 mRNA by MeRIP-qPCR in PBMCs from adult NHP gamma-ray TBI model (n = 6). e Validation of primers specificity and measurement of RNA m⁶A levels of NCOA4 mRNA by MeRIP-qPCR in HUVECs exposed to 10 Gy gamma rays at 1 h post-irradiation, using unirradiated samples as control group (n = 3). f The relative m⁶A levels of NCOA4 mRNAs in PBMCs from adult NHP gamma-ray TBI model exposed to 6.75 Gy gamma rays at 1, 7, 14, 21, and 28 days post-irradiation, using PBMCs from the unirradiated NHPs as control group (n = 6). g Temporal responding curves of the relative m⁶A levels of NCOA4 mRNA in HUVECs with various doses of gamma rays (0.2 to 10 Gy) and post-irradiation time (1, 3, 6, 12, 24, and 48 h) (n = 3/group, 49 groups). h The expression levels of NCOA4 in HUVECs with various doses of gamma rays (0.2 to 10 Gy) exposure at 1, 3, 6, 12, 24, and 48 h and without irradiation (n = 3/group, 49 groups). i Temporal responding curves of the relative m⁶A levels of NCOA4 mRNA in human peripheral blood cells with different doses of gamma rays (0.2 to 10 Gy) in vitro and post-irradiation time (1, 3, 6, 12, and 24 h) (n = 3/group, 41 groups). Data in a and e are presented as means ± SD and analyzed by two-sided Student’s t test. MeRIP-qPCR assays in f–i were conducted using NCOA4 primer 3. For data in f and h, one-way ANOVA was applied, and adjusted by Dunnett’s method. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. Source data are provided.