Fig. 2: REST induction in AD mice with early pathology.
From: A neurodegeneration checkpoint mediated by REST protects against the onset of Alzheimer’s disease
a–c REST induction in neurons that accumulate early tau pathology. a Immunolabeling of REST (green) the marker of early tau pathology, phospho-Ser202 tau (antibody CP13, red) and DNA (DAPI, blue) in the cortex (CTX) and the CA1 region of the hippocampus, shows elevated nuclear REST levels in neurons with accumulation of pSer202 tau in 11-month-old 3xTg mice b, c Quantification of nuclear REST levels in 11-month-old WT (n = 4) and 3xTg (n = 4) cortex (b) and 11-month-old WT (n = 4) and 3xTg (n = 6) hippocampus (c). d–f Loss of REST in aged 3xTg mice with advanced tau pathology. d Immunolabeling of REST (green), phospho-Ser396 tau, a marker of late, fibrillary, tau pathology (antibody PHF1, red) and DNA (DAPI; blue) in the cortex (CTX) and the CA1 region of the hippocampus, shows decreased nuclear REST levels in PHF1-positive neurons in 22-month-old 3xTg mice. Quantification of nuclear REST levels in the cortex (e) and hippocampus CA1 sector (f) of 22-month-old WT (n = 3) and 3xTg (n = 3) mice. g, h REST induction in J20 mice with early Aβ pathology. g Immunolabeling of REST (green), the neuron marker MAP2 (magenta) and nuclei (DAPI, blue) in the CA1 region of the hippocampus, shows elevated nuclear REST levels in 3-month-old J20 mice. h Quantification of nuclear REST levels in 3-month-old WT (n = 7) and J20 (n = 7) mice. i, j Loss of REST in aged J20 mice with advanced Aβ plaque pathology. i Immunolabeling of REST (green), Aβ (red) and DNA (DAPI, blue) in the CA1 region of the hippocampus, shows decreased nuclear REST levels in 18-month-old J20 mice. j Quantification of nuclear REST levels in 18-month-old WT (n = 6) and J20 (n = 6) mice. b, c, e, f, h, j Individual values (representing the average mean fluorescence intensities/mouse) as well as the mean ± S.E.M are shown. P-values were generated by one-way ANOVA with Tukey’s post-hoc test (b, c, e, f) or two-tailed unpaired t-test (h, j). a.u.-arbitrary units. Scale bars, 25 µm. Source data are provided as a Source Data file.
