Fig. 2: A2AR expression is elevated on tumor-infiltrating T cells and cDC2s. | Nature Communications

Fig. 2: A2AR expression is elevated on tumor-infiltrating T cells and cDC2s.

From: A2AR eGFP reporter mouse enables elucidation of A2AR expression dynamics during anti-tumor immune responses

Fig. 2

A WT, A2AR-/- or A2AR eGFP reporter mice were inoculated sub-cutaneously with 5 × 105 AT-3 ova tumors. A. Tumor size was measured over time (mm2). Data represent the mean ± SEM of 6 (WT) or 7 (A2AR-/-) mice per group. B–L Immune cells from draining lymph nodes and tumors were analyzed by flow cytometry at day 21 post-tumor inoculation unless otherwise stated. B Representative flow cytometry plots from concatenated samples. C, E Percentage of indicated cell types expressing GFP (A2AR) within DLNs (C) or tumors (E). D, F Proportion of all GFP+ cells that are of indicated lineage within DLNs (D) or tumors (F). C, D Data represent the mean ± SEM of 10 mice per group from 2 pooled experiments. E, F Data represent the mean ± SEM of 28 mice per group from 5 pooled experiments. G Relative expression of GFP (A2AR) for indicated cell types in the DLN and tumor. MFI was normalized such that expression in the DLN was equal to 1. Data represent the mean ± SEM of 10 mice per group from 2 pooled experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, unpaired two-sided t test. H MFI of GFP (A2AR) expression by each cell type isolated from AT3ova tumors. Data represented as mean ± SEM of 5 mice per group. ****p < 0.0001, statistics determined by one-way ANOVA I Expression of GFP (A2AR) in indicated cell types isolated from tumors at indicated timepoints. Numbers represent the GFP MFI. Data represent concatenated samples from n = 6 mice. J Expression of GFP (A2AR) in indicated myeloid populations. ΔGFP expression calculated as GFP MFI minus the background fluorescence for each cell type as observed in WT mice. Data represented as the mean ± SEM from 24 mice per group, pooled from 4 independent experiments. ****p < 0.0001, statistics determined by one way ANOVA. K Expression of MHCII and GFP on cDC2 cells (Gated as CD45+TCRβ-NK1.1-Ly6C-CD64-CD11c+MHCII+F4/80lowCD103-XCR1-). L Expression of MHCII, CD80, and CD86 on total cDC2 (Red), GFP- cDC2 (Blue) and GFP+ cDC2 (Green). K, L. Data concatenated from 6 individual mice. Source data are provided as a Source Data file.

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