Fig. 2: GlycDH catalyzed oxidation of O- and C-β-d-glucosides.
From: Enzymatic β-elimination in natural product O- and C-glycoside deglycosylation
a Reaction scheme. b–d Time courses of reactions with phlorizin (b), puerarin (c), and nothofagin (d). Conditions: 100 mM potassium phosphate, pH 5.7 (b, d), with 2.0 mM TCEP added (c). The substrate (2.0 mM) was incubated with K3[Fe(CN)6] (8.0 mM), DMSO (5.0 vol.%) and GlycDH (0.02, 0.25 and 0.10 mg/mL for b–d, respectively (n = 1 individual experiment). Source data are provided as a Source data file.
