Fig. 7: Expression of HAP2-2 during Lu. longipalpis infection. | Nature Communications

Fig. 7: Expression of HAP2-2 during Lu. longipalpis infection.

From: HOP1 and HAP2 are conserved components of the meiosis-related machinery required for successful mating in Leishmania

Fig. 7

A Abdominal midgut tissue after 5 days of infection showing expression of HAP2-2 in a minority of MA37 mNG::HAP2-2 promastigotes. DNA staining (Hoescht 33342, blue) differentiates the larger nucleus of insect cells and the smaller nucleus of promastigotes. Arrowheads point to cells expressing HAP2-2 in the cytoplasm, and white arrows indicate smaller, rounded cells with free flagella expressing HAP2-2 on the surface. B Abdominal midgut showing a sub-population of promastigotes attached to the midgut wall expressing HAP2-2, with diffuse and/or highly localized staining in the cytoplasm. C Higher magnification of a promastigote released from the midgut showing diffuse expression of HAP2-2 throughout the cell body, including the flagellum. D Abdominal midgut tissue with a high proportion of promastigotes expressing L747 mNG::HAP2-2 in the cytoplasm. Arrowheads indicate rounded cells expressing HAP2-2 either in the cytoplasm or on the surface, with elongated forms expressing it in the cytoplasm (white arrows). E Thoracic midgut after 5 days of infection showing expression of HAP2-2 in a majority of MA37 mNG::HAP2-2 promastigotes. Hoechst staining in blue, mNeonGreen in green. Scale bars are indicated in the DIC images. Infections and imaging were independently repeated at least twice, with the analysis of 3–5 midguts each time.

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