Table 1 L. tropica control, null mutants, and re-expressors used to analyze the essentiality of meiotic-related genes for hybridization in vitro

From: HOP1 and HAP2 are conserved components of the meiosis-related machinery required for successful mating in Leishmania

Parental strains		Frequency of hybrid recovery: #positive wells ^a/#total wells^b (%)	p-value^c
Crosses with fluorescent null mutants (Fig. 2B)
L. tropica MA37 eGFP-Neo	L. tropica L747 mCherry-Sat	40/72 (55.5%)	-
L. tropica MA37 eGFP-Neo	L. tropica L747 Δhop1 mCherry-Sat	4/72 (5.6%)	0.0583
L. tropica MA37 Δhop1 eGFP-Neo	L. tropica L747 mCherry-Sat	21/72 (29.2%)	<0.0001
L. tropica MA37 Δhop1 eGFP-Neo	L. tropica L747 Δhop1 mCherry-Sat	2/72 (2.8%)	<0.0001
L. tropica MA37 eGFP-Neo	L. tropica L747 Δspo11 mCherry-Sat	37/72 (51.4%)	0.9996
L. tropica MA37 Δspo11 eGFP-Neo	L. tropica L747 mCherry-Sat	42/72 (58.3%)	0.9994
L. tropica MA37 Δspo11 eGFP-Neo	L. tropica L747 Δspo11 mCherry-Sat	39/72 (54.1%)	0.9998
L. tropica MA37 eGFP-Neo	L. tropica L747 Δmnd1 mCherry-Sat	43/72 (59.7%)	0.7219
L. tropica MA37 Δmnd1 eGFP-Neo	L. tropica L747 mCherry-Sat	50/72 (69.4%)	0.9993
L. tropica MA37 Δmnd1 eGFP-Neo	L. tropica L747 Δmnd1 mCherry-Sat	40/72 (55.6%)	>0.9999
L. tropica MA37 eGFP-Neo	L. tropica L747 Δhop2 mCherry-Sat	24/72 (33.3%)	0.3938
L. tropica MA37 Δhop2 eGFP-Neo	L. tropica L747 mCherry-Sat	27/72 (37.5%)	0.165
L. tropica MA37 Δhop2 eGFP-Neo	L. tropica L747 Δhop2 mCherry-Sat	19/72 (26.4%)	0.0267
L. tropica MA37 eGFP-Neo	L. tropica L747 Δdmc1 mCherry-Sat	24/72 (33.3%)	0.8405
L. tropica MA37 Δdmc1 eGFP-Neo	L. tropica L747 mCherry-Sat	31/72 (43.1%)	0.1663
L. tropica MA37 Δdmc1 eGFP-Neo	L. tropica L747 Δdmc1 mCherry-Sat	25/72 (34.7%)	0.2271
L. tropica MA37 eGFP-Neo	L. tropica L747 Δhap2-1 mCherry-Sat	14/72 (19.4%)	0.0018
L. tropica MA37 Δhap2-1 eGFP-Neo	L. tropica L747 mCherry-Sat	13/72 (18.0%)	0.0029
L. tropica MA37 Δhap2-1 eGFP-Neo	L. tropica L747 Δhap2-1 mCherry-Sat	9/72 (12.5%)	0.0003
L. tropica MA37 eGFP-Neo	L. tropica L747 Δhap2-2 mCherry-Sat	1/72 (1.4%)	<0.0001
L. tropica MA37 Δhap2-2 eGFP-Neo	L. tropica L747 mCherry-Sat	0/72 (0%)	<0.0001
L. tropica MA37 Δhap2-2 eGFP-Neo	L. tropica L747 Δhap2-2 mCherry-Sat	0/72 (0%)	<0.0001
Crosses with non-fluorescent null mutants (Fig. S3B)
L. tropica MA37 SSU-Bsd	L. tropica L747 SSU-Pac	26/72 (36.1%)	-
L. tropica MA37 SSU-Bsd	L. tropica L747 Δhop1-Pac	0/72 (0%)	<0.0001
L. tropica MA37 SSU-Bsd	L. tropica L747 Δhap2-2-Pac	5/72 (6.9%)	0.0004
Crosses with re-expressors (Fig. 2E)
L. tropica MA37 eGFP-Neo	L. tropica L747 Δhop1::HOP1-Sat	32/72 (44.4%)	<0.0001
L. tropica MA37 Δhop1 eGFP-Neo	L. tropica L747 Δhop1::HOP1-Sat	28/72 (38.8%)	<0.0001
L. tropica MA37 eGFP-Neo	L. tropica L747 Δhap2-2::HAP2-2-Sat	21/72 (29.1%)	0.0002
L. tropica MA37 Δhap2-2 eGFP-Neo	L. tropica L747 Δhap2-2::HAP2-2-Sat	26/72 (36.1%)	<0.0001

^aWells considered positive when parasites grew in double drugged media (G418 and Nourseothricin) and expressed both eGFP and mCherry.
^bTotal positive wells calculated based on 3 independent experiments.
^cp-values < 0.05 are considered significant. For null mutant’s crosses, p-values were calculated relative to control crosses, and re-expressors with the relevant gene-deficient crosses. p-values determined by one-way ANOVA for multiple comparison of preselected pairs and a two-step step-up method of Benjamini, Krieger and Yekutieli to correct false-discovery.

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Table 1 L. tropica control, null mutants, and re-expressors used to analyze the essentiality of meiotic-related genes for hybridization in vitro

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