Fig. 5: LRRC8A deficiency impairs T cell-mediated antiviral immunity to LCMV Armstrong infection.

a Experimental design of LCMV Armstrong infection (2 × 10⁵ PFU). 10⁶ P14 cells isolated from WT (Lrrc8a^{f/f; P14}) or KO (Lrrc8a^{f/f, Cd4-Cre; P14}) mice (CD45.1⁺) were adoptively transferred to B6 mice (CD45.2⁺). b P14 T cells (Vα2⁺CD45.1⁺CD8⁺) in the spleen on the day 3 pi (n = 5 mice/group). c Virus load detected in the serum and spleen from mice in (b), measured by RT-qPCR (n = 5 mice/group). d Expression of Nur77 in P14⁺ T cells in spleen from mice in (b) (n = 5 mice/group). e Ki67 expression in P14 cells in the spleen from mice in (b) (n = 5 mice/group). f–g Evaluation of CD8⁺T cell effector/memory status. (f) CD127 and KLRG1 staining of P14 CD8⁺ T cells from mice in (a) on the day 14 pi. MPECs (CD127⁺KLRG1⁻) and SLECs (CD127^-KLRG1⁺) (n = 5 mice/group). g CD62L^hi memory T cells from mice in (f) (CD45.1⁺CD8⁺Vα2⁺CD44⁺CD62L⁺) on day 14 pi (n = 5 mice/group). h CD62L^hi memory T cells (CD45.1⁺CD8⁺Vα2⁺CD44⁺CD62L⁺) from mice in (a) on day 45 pi (n = 4 mice/group). Representative flow cytometry plots were shown on the right and their quantification on the left for (b, d–h). Data are representative of two (c, g, h) or three (b, d–f) independent experiments. Two-sided unpaired t test was used in (b–h), Data are presented as mean ± SEM. Source data are provided as a Source Data file.