Fig. 1: INPP5D expression in the human brain is restricted to microglia in the human brain. | Nature Communications

Fig. 1: INPP5D expression in the human brain is restricted to microglia in the human brain.

From: INPP5D regulates inflammasome activation in human microglia

Fig. 1

Human brain sections (25 μm) (a) and cultured iMGs (b) were immunostained for IBA1 and INPP5D. Nuclei are visualized with DAPI and preparations were imaged using confocal microscopy. Images representative of 16 human brain samples and over three iMG differentiations analyzed. Scale bars = 50 μm. c–e UMAP plots of sNucRNAseq of iMGs combined with snRNAseq of dorsolateral prefrontal cortex (dlpfc) from 12 human postmortem brain samples29 using Harmony35 to integrate across datasets. Single nucleus data from human brain samples and iMGs are depicted separately in (c) and (d), respectively. Relative INPP5D expression (after applying the SCTransformation in the Seurat package) across the harmonized iMG and human brain single nucleus samples shown in (e). Data are from 7121 iMG nuclei, 55,671 nuclei from the postmortem human brain (39,239 glutamatergic neurons, 14,958 astrocytes, 1474 microglia). f–i Data from microglia subcluster from the snRNAseq in c and d were isolated and re-clustered to examine microglial subsets. UMAP plots are shown in (f–h). Relative INPP5D expression (z-score of log-transformed, normalized data) across the subclusters is shown in (h). INPP5D was significantly lower in cluster 13 (adj. p-val = 8.0 × 10−226, Wilcoxon rank-sum test, with FDR correction). A dot plot showing a subset of the genes that define cluster 13 are shown in (i). See also Supplementary Fig. 1 and Supplementary Data 1 showing enrichments in all microglial clusters.

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