Fig. 4: Validation of m6A sites identified in MePMe-seq via SELECT in HeLa poly(A)+ RNA. | Nature Communications

Fig. 4: Validation of m6A sites identified in MePMe-seq via SELECT in HeLa poly(A)+ RNA.

From: MePMe-seq: antibody-free simultaneous m6A and m5C mapping in mRNA by metabolic propargyl labeling and sequencing

Fig. 4

a The normalized ΔCq values of SELECT qPCR measurements are shown for five sites located in a DRACH motif and three sites located in a non-DRACH motif. Mean values and SD from n = 5 biological replicates are shown. Statistical significance determined via one-sample one-tailed t test (n.s. P > 0.05; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001). The P values for – FTO versus + FTO samples are for MALAT1 2.6 × 10−5, for AHNAK 4.8 × 10−3, for MARCH6 6.9 × 10−3, for NFX1 9.8 × 10−4, for SRRM2 5.0 × 10−3, for WDR6 4.1 × 10−3, for CTNNB1 1.1 × 10−5 and for FLNB 0.39. b IGV browser coverage tracks of MePMe-seq data for the same sites from cells grown with PSH (purple) or methionine (gray) as control. Green bars indicate terminations identified by JACUSA2. c Comparison with m6A-SAC-seq, GLORI, eTAM-seq, MeRIP, SEAL, miCLIP, m6A CLIP, PA-m6A-CLIP, improved (imp.) m6A-seq, m6A-REF-seq, DART-seq or m6A-label-seq sequencing datasets64,81,82,91. Checkmark for sites present, x for sites not present in dataset (data obtained from literature). Source data are provided as a Source Data file.

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