Fig. 6: Termination signatures at different modified nucleosides after metabolic labeling. | Nature Communications

Fig. 6: Termination signatures at different modified nucleosides after metabolic labeling.

From: MePMe-seq: antibody-free simultaneous m6A and m5C mapping in mRNA by metabolic propargyl labeling and sequencing

Fig. 6

Two representative IGV coverage tracks of MePMe-seq data are shown for RNA modifications (a) m6A, (b) m5C, (d) Am, (e) Um, (f) Gm, (g) Cm and (h) m1A. poly(A)+ RNA from HeLa cells labeled with PSH (purple) or methionine as control (gray) was used. Terminations identified by JACUSA2 are indicated by a additional brackets. Numbers indicate reads and the calculated arrest rate (%) in that position. Arrow indicates orientation of coding strand. Box plots show bioinformatic analysis of termination signatures based on the coverage (Diff ctr-PSH) at the positions –2, –1, 0, 1, 2 of frequently identified modification sites of (a) m6A in mRNA, (c) Ψ, (d) Am, (e) Um, (f) Gm and (g) Cm (Nm in rRNA). In the boxplots the center lines, medians, upper and lower quartiles, whiskers (1.5×) and outliers are shown. Source data are provided as a Source Data file.

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