Fig. 7: Detection of m5C sites using MePMe-seq.
a Overlap of identified m5C sites between n = 2 MePMe-seq experiments (44% calculated from rep2 to rep1). b IGV browser coverage tracks of MePMe-seq data for the indicated FURIN and PXDN mRNAs from cells labeled with PSH (purple) or methionine as control (gray). Blue and green bars indicate terminations at C and A, respectively, identified by JACUSA2. Numbers (%) denote the calculated arrest rate in that position. Arrow indicates orientation of coding strand. c Frequency of m5C sites per transcript and replicate. d Metatranscript analysis showing a density plot of the distribution of m5C sites detected by MePMe-seq. e Consensus motif for sequences surrounding identified m5C sites. Representative example of n = 2 biologically independent samples. f Overlap of m5C identified in MePMe-seq per replicate (combined sites from n = 2 independent experiments with HS filtering) with sites identified by bisulfite sequencing (BS-seq), improved BS-seq, Aza-IP and miCLIP81. g Overlap of m5C sites identified in MePMe-seq (combined sites from n = 2 independent experiments) with all m5C sites from ATLAS database81, when increasing uncertainty region around the site is applied.
