Fig. 1: NULISA design and proof of concept.
From: NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing
a Schematic of the NULISA workflow. (1) Immunocomplex formation; (2) first capture of immunocomplexes to dT beads; (3) bead washing to remove unbound antibodies and sample matrix components; (4) release of immunocomplexes into solution; (5) recapture of immunocomplexes onto streptavidin beads; (6) bead washing and DNA strand ligation to generate reporter DNA; (7a) detection and quantification of reporter DNA levels by qPCR; (7b) quantification of reporter DNA levels by NGS. b Standard curves for IL4 detection generated following the traditional PLA (red line) or NULISA (blue line) protocols using the same set of reagents. The serial dilution of the standard spanned from 200 pM to 10 aM. Error bars represent mean +/− one standard deviation (n = 6). c Sensitivity and dynamic range comparison between NULISA (blue line) and SIMOA (red line) using the same pair of antibodies to detect HIV p24. Error bars represent mean +/− one standard deviation (n = 3). Source data are provided as a Source data file.
