Fig. 2: NULISAseq sensitivity and correlation across multiplex levels.

a Comparison of the sensitivity of 200-plex and single-plex assays for LIF, IL5, and IL13 detection. Error bars represent mean +/− one standard deviation (n = 3). b Pearson correlation of protein levels measured using 200-plex and single-plex assays with 12 individual healthy donor plasma samples. The 200-plex data were normalized using internal and inter-plate controls and then log2-transformed to yield NULISA Protein Quantification (NPQ) units. Single-plex data in absolute concentration (aM) were also log2-transformed. Least-squares regression lines are shown on the plots. Two-sided tests were carried out to assess whether correlation coefficients significantly differ from zero; unadjusted p-values are shown. c Pearson correlation of protein levels measured using 200-plex and 24-plex NULISA methods with the same 12 plasma samples. Data were normalized using internal and inter-plate controls and then log2-transformed to yield NULISA Protein Quantification (NPQ) units. Least-squares regression lines are shown on the plots. Two-sided tests were carried out to assess whether correlation coefficients significantly differ from zero; unadjusted p-values are shown. The same antibody concentrations were used in all the assays. Source data are provided as a Source data file.