Fig. 1: scRNA-seq analysis of Gli1-expressing cells.

a Scheme of the experimental strategy of scRNA-seq. b Visualization of unsupervised clustering in a t-distributed stochastic neighbor embedding (t-SNE) plot of 8508 Gli1⁺ cells isolated from hindlimb skeletal muscles (n = 3). c Each cell clustering percentage in t-SNE plot of 8,508 cells. Percentage of cells is shown in the pie diagrams. d Heatmap of scRNA-seq data showing enriched genes in the 7 different clusters. e Violin plots showing the expression levels of representative marker genes across the 7 clusters. f Distribution of Pax7 across all populations. g Scheme of the experimental strategy. h Visualization of unsupervised clustering in a t-distributed stochastic neighbor embedding (t-SNE) plot of 17,959 MuSCs isolated from hindlimb skeletal muscles (n = 3). i Distribution of Gli1 expression across Pax7⁺ populations.