Fig. 3: Usp30 KO prevents loss of striatal TH+ dopaminergic fibers and preserves dopamine and its metabolites in the α-synuclein-based mouse model.
From: Knockout or inhibition of USP30 protects dopaminergic neurons in a Parkinson’s disease mouse model
a Representative striatal sections of TH immunohistochemistry in male mice at 28 weeks post-injection of AAV-A53T-SNCA.; enlarged in lower panels. Scale bar, 1 mm for upper panels, 100 µm for lower panels. b Relative optical density of TH+ fibers in the ipsilateral striatum compared with the contralateral side of male mice in each group (n = 7 for WT group, n = 8 for mito-QC group, n = 7 for mito-QC/Usp30 KO group). Significance determined by one-way ANOVA. Error bars represent mean ± s.d.; ****P < 0.0001. c Mean dopamine levels (ng/mg protein) in the ipsilateral striatum of female (open dots) and male (closed dots) mice in each group (n = 14 for WT + EV, n = 10 for mito-QC + EV, n = 15 for mito-QC/Usp30 KO + EV, n = 16 for WT + SNCA, n = 14 for mito-QC + SNCA, n = 13 for mito-QC/Usp30 KO + SNCA groups of mice). Significance determined by one-way ANOVA. Error bars represent mean ± s.d.; ***P < 0.001, ****P < 0.0001. d Mean of 3,4-Dihydroxyphenylacetic acid levels (DOPAC, ng/mg protein) in the ipsilateral striatum of female (open dots) and male (closed dots) mice in each group (n = 14 for WT + EV, n = 10 for mito-QC + EV, n = 15 for mito-QC/Usp30 KO + EV, n = 16 for WT + SNCA, n = 14 for mito-QC + SNCA, n = 13 for mito-QC/Usp30 KO + SNCA groups of mice). Significance determined by one-way ANOVA. Error bars represent mean ± s.d.; ****P < 0.0001. e Mean levels of homovanillic acid (HVA, ng/mg protein) in the ipsilateral striatum of female (open dots) and male (closed dots) mice in each group (n = 14 for WT + EV, n = 10 for mito-QC + EV, n = 15 for mito-QC/Usp30 KO + EV, n = 16 for WT + SNCA, n = 14 for mito-QC + SNCA, n = 13 for mito-QC/Usp30 KO + SNCA groups of mice). Significance determined by one-way ANOVA. Error bars represent mean ± s.d.; ****P < 0.0001; *P < 0.05. f Mean levels of 3-methoxytyramine (3-MT, ng/mg protein) in the ipsilateral striata of female (open dots) and male (closed dots) mice in each group (n = 14 for WT + EV, n = 10 for mito-QC + EV, n = 15 for mito-QC/Usp30 KO + EV, n = 16 for WT + SNCA, n = 14 for mito-QC + SNCA, n = 13 for mito-QC/Usp30 KO + SNCA groups of mice). Significance determined by one-way ANOVA. Error bars represent mean ± s.d.; **P < 0.01. Source data are provided as a Source Data file.
