Fig. 6: SvCslG increases the production of QA-C3-GlcA. | Nature Communications

Fig. 6: SvCslG increases the production of QA-C3-GlcA.

From: Deciphering triterpenoid saponin biosynthesis by leveraging transcriptome response to methyl jasmonate elicitation in Saponaria vaccaria

Fig. 6

Different OD levels of Agrobacterium tumefaciens harboring SvCslG were individually co-infiltrated with A. tumefaciens harboring SvβAS, SvC28, SvC16 and SvC23-1 (a) and SvC23-2 (b). Error bars indicate mean ± SD (n = 3 biologically independent samples). Analysis calculated using a one-way ANOVA test revealed a significant effect of SvCslG strain OD level on the production of QA-C3-GlcA [(a), F(1, 4) = 14.27, p = 0.0195; (b), F(2, 6) = 8.98, p = 0.0157]. Asterisks indicate statistical significance with a Tukey HSD test ((a) p = 0.019; (b) p = 0.014). Production of GA-C16-OH-C3-GlcA was not significantly affected by the OD level of SvCslG strain [(a), F(1,4) = 6.39, p = 0.0648; (b), F(2,6) = 1.988, p = 0.218]. Source data and additional p-values for Tukey tests are provided as a Source Data file. Compound verification by LC-MS and mass spectra are shown in Supplementary Fig. 14.

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