Fig. 6: PpROP4 localization is altered in ropgap, ren mutants.

a Localization of PpROP4-mNG in WT and ropgap, ren mutant tip cells. Note that the boundary (brackets) between the apical dome and subapical membrane is less obvious in mutant cells. Scale bar: 10 µm. b Representative intensity plots of PpROP4 along the apical membrane. Intensities were measured along the blue lines shown in (a). The dome region is marked by pink lines. c Quantification of the intensity ratio of peak and base in the plots. The number of cells used for quantification is shown at the bottom. Data are presented as mean values ± SD with individual points shown. The comparison was performed using a two-tailed student’s t-test. ***p < 0.001. The exact p-values are available in Source Data. d Early accumulation of PpROP4 at branching sites in subapical cells (indicated by stars) of ropgap, ren mutants. Images were taken starting from the nuclear envelope breakdown. Scale bar: 10 µm. Similar results in (d) were obtained in three independent experiments. Source data are provided as a Source Data file.