Fig. 3: Evaluation on the TNBC-targeting effect of DNA-PAE@BAY-876 in vitro. | Nature Communications

Fig. 3: Evaluation on the TNBC-targeting effect of DNA-PAE@BAY-876 in vitro.

From: Inhibition of glycolysis-driven immunosuppression with a nano-assembly enhances response to immune checkpoint blockade therapy in triple negative breast cancer

Fig. 3

a Flow cytometric analysis on the cell binding behavior of aptPD-L1 to 4T1/HC11 cells under pH 7.4. b Cell binding behavior of aptCTLA-4 to activated/inactivated T cells under pH 7.4. c Interaction between 4T1 or HC11 cells and DNA-PAE nanoassemblies under different environmental pH conditions at 37 °C for 2 h. d DNA-PAE@BAY-876-medited GLUT1 inhibition in 4T1 cells and HC11 cells at 37 °C for 24 h. e, f Dose-dependent toxicity of DNA-PAE@BAY-876 to 4T1 cells and HC11 cells at 37 °C for 24 h. g, i Toxicity of BAY-876 to major immune cell populations at different pH conditions. (I) pH 7.4+activation, (II) pH 7.4+inactivation, (III) pH 6.8+activation, (IV) pH 6.8+inactivation. j, k Immunofluorescence analysis of GLUT1 and GLUT3 in 4T1 tumors. l, m Glucose uptake of 4T1 cells and T cells in the co-incubation system after treatment with BAY-876 or KL-11743. Flow cytometry and immunofluorescence experiments in panels a–d, j and k were repeated three times independently with similar results. Data are presented as mean values ± SEM (n = 3 biologically independent samples for panels e–i, l and m). Statistical analysis was carried out via two-way ANOVA method. Source data are provided as a Source Data file.

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