Fig. 4: DNA-PAE@BAY-876 treatment mounted potent antitumor effect in 4T1-splenic immune cell co-incubation system. | Nature Communications

Fig. 4: DNA-PAE@BAY-876 treatment mounted potent antitumor effect in 4T1-splenic immune cell co-incubation system.

From: Inhibition of glycolysis-driven immunosuppression with a nano-assembly enhances response to immune checkpoint blockade therapy in triple negative breast cancer

Fig. 4

a Apoptosis ratio of 4T1 cells in the co-incubation system after different treatment at 37 °C for 24 h. Apoptosis effect of 4T1 cells with pH 7.4 (b) and pH 6.8 (c) by western blotting at 37 °C for 24 h. (I) NC, (II) PAE, (III) aptCTLA-4, (IV) aptPD-L1, (V) BAY-876, (VI) DNA-PAE, (VII) DNA-PAE@BAY-876. Note: the molecular weight of Bcl-2 is 26 KDa; the molecular weight of Bax is 21 KDa; the molecular weight of Tubulin is 50 KDa. d, e Glucose uptake by 4T1 cells or T cells after different treatments under co-culture condition at 37 °C for 24 h. f, g Flow cytometric analysis on the expansion of CD4+/CD8+ T cells, IFN-γ+CD8+ T cells in the co-incubation system after different treatment at 37 °C for 24 h. h Statistical analysis of data shown in panels f and g by flow cytometry. (1) NC, (2) PAE, (3) aptCTLA-4, (4) aptPD-L1, (5) BAY-876, (6) DNA-PAE, (7) DNA-PAE@BAY-876. Flow cytometry and western blot experiments in panels a–c, f and g were repeated three times independently with similar results. Data are presented as mean values ± SEM (n = 3 biologically independent samples for panels d, e and h). Statistical analysis was carried out via two-way ANOVA method. Source data are provided as a Source Data file.

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