Fig. 3: ASO-mediated inhibition of mTORC2 reduces KA-induced seizures.
From: Targeted suppression of mTORC2 reduces seizures across models of epilepsy
mTORC2 activity in Rictor-ASO treated naïve mice. Representative western blots (a) and quantification of Rictor (b, t = 5.016 P < 0.001) and p-AktS473 (c, U = 1, P < 0.001) in control-ASO and Rictor-ASO treated mice (n = 8 per group). KA-induced behavioral seizures in Rictor-ASO treated mice. Racine score (d, U = 15, P = 0.033) and dose to elicit stage 4 seizure (e, U = 8, P = 0.004) in control-ASO (n = 9) and Rictor-ASO (n = 8) treated mice. KA-induced acute EEG seizures in control-ASO (n = 9) and Rictor-ASO (n = 8) treated mice (t = 4.059, P < 0.001). Representative EEG traces (f) and number of EEG seizures (g) from control-ASO and Rictor-ASO treated mice. Scale bars: X = 10 s, Y = 1 mV. Statistical tests used for analysis were two-tailed unpaired t-tests for normally distributed data or Mann-Whitney test for non-normally distributed data at a 95% confidence level. Data are represented as means ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file.
