Fig. 5: Nav1.2 is regulated by Rictor and binds to the mTORC2 components Rictor and Sin1.
From: Targeted suppression of mTORC2 reduces seizures across models of epilepsy
a Volcano plot showing differentially regulated phosphorylation events in Pten-Rictor fb-DKO (n = 6) compared to Pten-Rptor fb-DKO (n = 4) samples (Threshold P < 0.05). b Pathway enrichment analysis for phenotype (Monarch MPO) and cellular component (GO component) in downregulated phosphorylation events in Pten-Rictor fb-DKO compared to Pten-Rptor fb-DKO samples (FDR < 1%). Pull-down assays from HEK 293 T cells expressing GFP or GFP-Scn2a alone and co-expressed with HA-Sin1. c Representative western blots of lysates and pull-downs for GFP, Raptor, Rictor and β-actin from GFP pull-downs. d Representative western blots of lysates and pull-downs for GFP, HA, Raptor, Rictor and β-actin from HA pull-downs. Data shown are representative of 3 independent experiments. Source data are provided as a Source Data file.
