Fig. 3: Combined pSer:alum approach with either SMNP or 3M-052 induce enhanced antibody responses and promote bone marrow plasma cell responses.
a Full time course of the area under the curve (AUC) of total Env-binding serum IgG, as determined by ELISA. b AUC of Env-binding serum IgG at week 2. c AUC of Env-binding serum IgG at week 6. d Percentage of Env-binding AUC retained at week 24 relative to week 12. Percentage retained is calculated by taking the AUC at week 12 over the AUC at week 24. All other pairwise comparisons were not significant. e BG505 Env-specific bone marrow plasma cell (BM BPC) responses from bone marrow aspirates as determined by ELISpot assays. Env-specific ELISpots were performed at week 36. f Env-specific ELISpots performed at week 49. Black triangles and gray dotted lines represent time of immunization. Mean and SEM or geometric mean and geometric SD are plotted depending on the scale in all figures unless otherwise stated. For Fig. 3a–f, n = 6 per group. Statistical significance for a–d were measured using either Ordinary one-way ANOVA with Tukey’s multiple comparisons test or Kruskal-Wallis test with Dunn’s multiple comparisons test, depending on the distribution of the data (determined by normality or lognormality tests). Statistical significance for BM BPC ELISpots (e, f) was tested using either unpaired two-tailed Mann-Whitney tests or Kruskal-Wallis test with Dunn’s multiple comparisons test, depending on the objectives of the study. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data File.
