Fig. 5: pSer:alum-mediated antigen delivery and SMNP adjuvant promote non-base-binding Env-specificities.

a Antibody responses to the base of the trimer from serum at week 10, determined by cross-competition ELISA to 19R. Percentage of binding retained after addition of 19R is calculated by taking the AUC with 19R over the AUC without 19R. b Antibody responses to the base of the trimer, measured from serum at week 12. c Quantification of epitope sites on the Env trimer recognized using EMPEM, per animal group (out of 5 unique epitopes detected across all study groups), based on 3D maps presented in Supplementary Fig. 7a. d Total number of total epitope sites on the Env trimer recognized in EMPEM per animal, based on 3D maps presented in Supplementary Fig. 7a. e Flow cytometry gating of Env-specific (Env-AF647⁺Env-BV421⁺) and non-base-binding Env-specific (Env-AF647⁺Env-BV421⁺Env-bKO-PE⁺) memory B cells (B_Mem). f Frequency of Env-binding (Env⁺) cells as a percentage of total B cells. g Frequency of non-base-binding (Env⁺Env-bKO⁺) B_Mem cells as a percentage of total B cells. EMPEM data (Fig. 5c-d) from Group 1, alum, have been previously published²¹. For Fig. 5a, b, f, g, n = 6 per group. Statistical significance for cross-competition ELISAs (a-b) was tested using Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Statistical significance for EMPEM and B_Mem cell frequency (c-g) was tested using either unpaired two-tailed Mann-Whitney tests or Kruskal-Wallis test with Dunn’s multiple comparisons test, depending on the objectives of the study. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data File.