Fig. 1: Identification and positional cloning of rin1.
From: Soybean reduced internode 1 determines internode length and improves grain yield at dense planting
a Whole-chromosome scan of QTLs for plant height both in F2 (Harbin, 2017) and F3 (Harbin, 2018) populations. The red dotted line represents the threshold for QTL detection, LOD (F2) = 4.3, LOD (F3) = 3.2. b Whole-chromosome scan of QTLs for internode length in both F2 and F3 populations. The red dotted line represents the threshold for QTL detection. LOD (F2) = 3.6, LOD (F3) = 3.4. c Schematic illustration of the chromosomal location of the QTL on chromosome 12. d Fine-mapping of rin1 to a 76.31 kb region. Characterization of key recombinants in the immediate vicinity of the RIN1 locus showing recombination break points (left panel). A: Homozygous for the allele from rin1; B: homozygous for the allele from HH43; H, heterozygous. Segregation of internode length is shown in boxplot format (right), wherein the interquartile region, median and range are represented by the box, bold vertical line, and horizontal line, respectively. All the plants used for phenotypic measurements in (d) were planted in a field in Harbin, China (45°75′N, 126°63′E) and the phenotypes were scored after maturity. n represents the number of plants of each genotype. e Gene structures of the rin1 candidate gene show three allelic variations in Wm82, HH43, HN35 and rin1. +, coding regions (CDS). AA, Amino acid. The pink bars represent Protein kinase domain, green bars represent Coiled-coil domain and blue bars represent WD40-Repeat domain. The triangle symbol represents the base mutation position. Source data of (d) are provided as a Source Data file.
