Fig. 4: RIN1 regulates internode length through gibberellin metabolism.
From: Soybean reduced internode 1 determines internode length and improves grain yield at dense planting
a Relative expression levels of GA2ox7a (top) and GA2ox7b (bottom) in NIL-RIN1HH43 and NIL-rin1 under LD conditions (16 h light/8 h dark) in a growth chamber. b Relative expression levels of the GA2ox7a (top) and GA2ox7b (bottom) genes in Wm82 and rin1CR1, rin1CR2 mutants under LD conditions (16 h light/8 h dark) in a growth chamber. Data shown in (a, b) are relative to the control gene Tubulin (TUB). Data shown in (a, b) are means ± SD from three independent biological replicates. A student’s t-test (two-sided) was used to generate the P-values of (a, b), *P < 0.05, **P < 0.01. c Constructs used for the transient transfection assay. p35S, 35S Promoter. pGA2ox7a, GA2ox7a Promoter. pGA2ox7b, GA2ox7b Promoter. d Luciferase (LUC) activity under the control of the GA2ox7a or GA2ox7b promoter, the combinations in (d) link to the numbers in (c). Data are means ± SD from three independent biological replicates. One-way ANOVA with Tukey’s post-test was performed to determine statistically significant differences, P < 0.05. a–c letters indicate statistically significant differences. e Relative expression levels of the GA2ox7a (top) and GA2ox7b (bottom) genes in Wm82, stf1 stf2 double mutants and GmSTF2-OE1 under LD conditions (16 h light/8 h dark) in growth chamber. Data shown are relative to the control gene Tubulin (TUB). Data shown are relative to the control gene Tubulin (TUB). Data shown are means ± SD from three independent biological replicates. A student’s t-test (two-sided) was used to generate the P-values, *P < 0.05, **P < 0.01. All the NIL-RIN1HH43 and NIL-rin1, Wm82, stf1 stf2 double mutants and GmSTF2-OE1 lines used for RT-qPCR in (a, b–e) were taken from the stem tips of seedlings at 10 DAE under LD incubators (16 h light /8 h dark). f GA1 (left) and GA8 (right) contents in NIL-RIN1HH43 and NIL-rin1 backgrounds determined by LC–MS/MS. Data shown are means ± SD from three independent biological replicates. A student’s t-test (two-sided) was used to generate the P-values, *P < 0.05, **P < 0.01. g Phenotypes of NIL-RIN1HH43 and NIL-rin1 seedlings treated with 20 μM GA3 at the V1 stage (one unrolled trifoliate leaf) (top) and after a week (bottom). Scale bar = 5 cm. h Internode lengths of seedlings treated with different concentrations of GA3. Data shown are means ± SD from three independent biological replicates. A student’s t-test (two-sided) was used to generate the P-values, *P < 0.05, **P < 0.01. Source data are provided as a Source Data file.
