Fig. 2: Neutron reflectometry. | Nature Communications

Fig. 2: Neutron reflectometry.

From: Myr-Arf1 conformational flexibility at the membrane surface sheds light on the interactions with ArfGAP ASAP1

Fig. 2

Component volume occupancy (CVO) profiles of membrane-associated myr-Arf1 and constituents of sparsely-tethered lipid bilayer membranes (stBLMs) composed of A PC:PS (85:15), B PC:PI(4,5)P2 (95:5), and C PC:PS:PI(4,5)P2 (87:10:3). The membrane coverage of Arf1 varies with the lipid composition. The shapes of the Arf1 profiles are similar: Arf1 associates peripherally with the lipid membrane, is primarily located in the membrane-adjacent bulk water phase, and the CVO profile peaks at ~45 Å from the bilayer center. Reflectivity curves and fit parameters for all stBLMs are provided in the Supplementary Information (Supplementary Figs. 4–6, Supplementary Table 1). Source data are provided as a Source data file.

Back to article page