Fig. 2: Basic Photophysical Properties of Lyso700D.
From: Adoptive macrophage directed photodynamic therapy of multidrug-resistant bacterial infection
a The normalized absorption and emission spectrums of Lyso700D in dimethyl sulfoxide (DMSO) with 0.1% trifluoroacetic acid (TFA). b Fluorescence intensity of Lyso700D at 700 nm against pH in Phosphate buffer saline (PBS). c Fluorescent images of Lyso700D aqueous solution versus different pH. d Basic photophysical properties of Lyso700D. a All the properties were tested in DCM; b maximum absorption wavelength, in nm; c maximum emission wavelength, in nm; d molar absorption coefficient, M−1 cm−1; e absolute fluorescence quantum yield; f luminescence lifetimes, in ns; g singlet oxygen quantum yield with MB as standard (ΦΔ = 56%); h intrinsic triplet excited-state lifetime, in µs. e 1O2 generation of Lyso700D trapped with DPBF in DMSO (with or without 0.1% TFA) irradiated with 740 nm LED light. f ns-TA spectra of Lyso700D in 0.1% TFA DCM after pulsed excitation, λex = 532 nm. Inset: Decay trace of Lyso700D in 684 nm. g ns-TA spectra of Lyso700D in DCM after pulsed excitation, λex = 532 nm. Inset: Decay trace of Lyso700D in 620 nm.
