Fig. 3: Cryo-EM structure of ATP-bound BSEPE1244Q.
From: Structural basis of bile salt extrusion and small-molecule inhibition in human BSEP
a Cryo-EM density map and model of ATP-bound BSEPE1244Q. The N-terminal half (TMD1 and NBD1) is colored orange and the C-terminal half (TMD2 and NBD2) is green. ATP and Mg2+ ions are shown as spheres. b Cartoon representation of NBDs viewed from the lipid membrane towards the cytoplasm. c, d Molecular interactions of ATP at NBS, shown together with the density of Mg2+-ATP (blue density). e, f 2D diagrams of the interactions of Mg2+-ATP at NBS1 and NBS2, respectively. These conserved motifs (A-loop, Q-loop, Signature motif, Walker-A, and Walker-B) are indicated in (c–f).
