Fig. 5: Tau mobility is sensitive to 1,6-HD.

a, b Representative Tau-mEos2 trajectories generated using segNASTIC showing instantaneous diffusion coefficients (the color bar represents log₁₀[μm²s^-1]) in an (a) synaptic region and (b) an axonal region, determined by VAMP2-pHluorin intensity (inset), of neurons treated with control vehicle (DMSO) or with 1,6-HD (2.5%). c Average MSD of Tau-mEos2 presynaptic trajectories from neurons treated with control vehicle or with 1,6-HD. d Corresponding areas under the MSD curves (AUC). e Average MSD of Tau-mEos2 axonal trajectories from neurons treated with control vehicle or with 1,6-HD. f Corresponding areas under the MSD curves (AUC). g Representative segNASTIC trajectories showing instantaneous diffusion coefficients from Tau-mEos2, and Tau LLPS mutants Tau^{I277P / I308P}-mEos2 and ΔTau74-mEos2. h, j Average MSD of the trajectories of mEos2-tagged Tau WT and LLPS Tau mutants in (h) presynapses and (j) axons. i, k Corresponding areas under the curve (AUC), in the (i) presynapses and (k) axonal compartment. Data in (c) to (f) and (h) to (k) are displayed as mean ± SEM. Data in (c, d) was obtained from n = 9 synapses from 9 different neurons. Data in (e, f) was obtained from n = 7 and 5 axons from different neurons. Data in (h) to (k) was obtained from n = 14 presynapses from 14 neurons and 18 axons from 18 neurons in Tau^WT, n = 11 synapses and axons from 11 neurons in Tau^{I277P / I308P}, and n = 17 synapses from 17 neurons and 12 axons from 12 neurons in ΔTau74. Data was obtained from 2 independent neuronal cultures. Statistical comparisons in (d) and (f) were performed using the unpaired two-tailed Student’s t-test with Welch’s correction in (d) and (f), or using the one-way ANOVA test in (i) and (k). Source data are provided as a Source Data file.